https://www.selleckchem.com/products/sar131675.html SDG on time.Chloroplast manganese transporter 1 (CMT1) and photosynthesis-affected mutant 71 (PAM71) are two membrane proteins that function sequentially to mediate the passage of manganese across the chloroplast envelope and the thylakoid membrane. CMT1 and PAM71 belong to a small five-member protein family in Arabidopsis thaliana. The other three, photosynthesis-affected mutant 71 like 3 (PML3), PML4 and PML5 are not predicted to reside in chloroplast membranes. In this study, the subcellular localization of PML3GFP, PML4GFP and PML5GFP was determined using transient and stable expression assays. PML3GFP localizes to the Golgi apparatus, whereas PML4GFP and PML5GFP are found in the endoplasmic reticulum. We also examined patterns of PML3, PML4 and PML5 promoter activity. Although the precise expression pattern of each promoter was unique, all three genes were expressed in the leaf vasculature and in roots. Greenhouse grown single mutants pml3, pml4, pml5 and the pml4/pml5 double mutant did not exhibit growth defects, however an inspection of the root growth revealed a difference between pml3 and the other genotypes, including wild-type, in 500 µM manganese growth conditions. Strikingly, overexpression of PML3 resulted in a stunted growth phenotype. Putative functions of PML3, PML4 and PML5 are discussed in light of what is known about PAM71 and CMT1.Since high quality natural aggregates are becoming scarce, it is important that industrial recycled products and by-products are used as aggregates for concrete. In Japan, the use of recycled aggregate (RG) is encouraged. Since, strength and durability of recycled aggregate concrete is lower than that of normal aggregate concrete, the use of recycled aggregate has not been significant. In order to improve physical properties of concrete using recycled coarse aggregate, blast furnace slag sand has been proposed. Recently, blast furnace slag sand is expected to improve