https://www.selleckchem.com/products/pf-06424439.html Bisphenol A, an endocrine disrupting compound, is widely used in food and beverage packaging, and it then leaches in food and source water cycles, and thus must be monitored. Here, we report a simple, low-cost and sensitive electrochemical sensor using graphene oxide and β-cyclodextrin functionalized multiwalled carbon nanotubes for the detection of BPA in water. This sensor electrode system combines the high surface area of graphene oxide and carbon nanotubes, and the superior host-guest interaction capability of β-cyclodextrin. A diffusion-controlled oxidation reaction involving equal numbers of protons and electrons facilitated the electrochemical sensing of BPA. The sensor showed a two-step linear response from 0.05 to 5 μM and 5-30 μM with a limit of detection of 6 nM. The sensors also exhibited a reproducible and stable response over one month with negligible interference from common inorganic and organic species, and an excellent recovery with real water samples. The proposed electrochemical sensor can be promising for the development of simple low-cost water quality monitoring system for monitoring of BPA in water.Bottom-up proteomics is a mainstay in protein identification and analysis. These studies typically employ proteolytic treatment of biological samples to generate suitably sized peptides for tandem mass spectrometric (MS) analysis. In MS, fragmentation of peptides is largely driven by charge localization. Consequently, peptides with basic centers exclusively on their N-termini produce mainly b-ions. Thus, it was long ago realized that proteases that yield such peptides would be valuable proteomic tools for achieving simplified peptide fragmentation patterns and peptide assignment. Work by several groups has identified such proteases, however, structural analysis of these suggested that enzymatic optimization was possible. We therefore endeavored to find enzymes that could provide enhanced activit