https://www.selleckchem.com/products/nhwd-870.html An electronic biosensor for odors was assembled by immobilizing the silk moth Bombyx mori pheromone binding protein (BmorPBP1) on a reduced graphene oxide surface of a field-effect transistor. At physiological pH, the sensor detects the B. mori pheromones, bombykol and bombykal, with good affinity and specificity. Among the other odorants tested, only eugenol elicited a strong signal, while terpenoids and other odorants (linalool, geraniol, isoamyl acetate, and 2-isobutyl-3-methoxypyrazine) produced only very weak responses. Parallel binding assays were performed with the same protein and the same ligands, using the common fluorescence approach adopted for similar proteins. The results are in good agreement with the sensor's responses bombykol and bombykal, together with eugenol, proved to be strong ligands, while the other compounds showed only poor affinity. When tested at pH 4, the protein failed to bind bombykol both in solution and when immobilized on the sensor. This result further indicates that the BmorPBP1 retains its full activity when immobilized on a surface, including the conformational change observed in acidic conditions. The good agreement between fluorescence assays and sensor responses suggests that ligand-binding assays in solution can be used to screen mutants of a binding protein when selecting the best form to be immobilized on a biosensor.In this paper, a novel gold film-coated V-shape dual-core photonic crystal fiber (V-DC-PCF) polarization beam splitter (PBS) based on surface plasmon resonance effect is proposed. The coupling lengths of the X-polarization (X-pol) and Y-polarization (Y-pol) and the corresponding coupling length ratio of the proposed V-DC-PCF PBS without gold film and with gold film are compared. The fiber structure parameters and thickness of the gold film are optimized through investigating their effects on the coupling lengths and coupling length ratio. As the propagation l