https://www.selleckchem.com/products/Cladribine.html Since its introduction in the 1970s, Fluorescence Correlation Spectroscopy (FCS) has become a standard biophysical and physical chemistry tool to investigate not only a diffusion but also a broad range of biochemical processes including binding kinetics and anomalous diffusion. Since the derivation of FCS equations for many biochemical processes shares many common steps with the diffusion FCS equation, it is important to understand the mathematical theory behind the diffusion FCS equation. However, because the derivation of FCS equations requires advanced Fourier Transform and inverse Fourier Transform theory, which most biologists and biochemists are not familiar with, it is often treated as a black box in practice. In this study, we provide a simple and straightforward step-by-step derivation of FCS equations for free diffusion based on calculus-level mathematics, so that FCS equations and its applications can be accessible to a broad audience. Additionally, we compare our derivation with the conventional Fourier Transform and inverse Fourier Transform theory based approach.Mercury is widely found in nature, however, in low concentrations, but anthropological activities have increased its concentration considerably. This causes various environmental hazards and human health. Many substances are capable of reversing the toxicity of mercuric chloride in the environment. The aim of the present study was to determine the chelating effect of vanillin, as well as to evaluate its capacity for cytoprotection in prokaryotic and eukaryotic plant models. Chelating activity was determined from vanillin's ability to reduce iron III ions. To evaluate cytoprotection in a unicellular prokaryotic and eukaryotic model, Escherichia coli and Candida albicans, respectively, were used. And to evaluate the cytoprotective activity in vegetables, lettuce seeds were submitted to different concentrations of mercuric chloride and its assoc