The aim of this paper was to identify the core microbes of producing lignocellulolytic enzymes during rice straw composting with functional bacterial agents inoculation. The results indicated that inoculation functional bacterial agents accelerated the degradation of organic matter and coarse fiber content by 7.58%, 8.82%, which were due to the fact that key enzymes and core microbes were stimulated. In addition, inoculation have reconstructed core microbes of producing lignocellulase. Meanwhile, inoculation functional bacterial agents not only as core bacteria to produce cellulase, xylanase and manganese peroxidase (MnP), but also increased most core microbial abundance. Redundancy analysis indicated that CMCase, xylanase, total nitrogen and MnP as key factors to affect the degradation of organic fractions in the core bacterial communities, while in the core fungal communities, were mainly affected by environmental factors (except for MnP). This study provided a theoretical basis for the efficiently degradation during agricultural wastes composting.Background Factors and policies which potentially explain the changes in alcohol consumption and related harms from 2010 to 2017 in 11 middle-income countries in the South-East Asian region (Cambodia, Lao PDR, Indonesia, the Philippines, Malaysia, Maldives, Myanmar, Sri Lanka, Thailand, Timor-Leste, and Vietnam) were examined. Methods Using secondary data from UN agencies, we analyzed trends in alcohol consumption, alcohol-attributable deaths and the burden of disease. Results Starting from a level of consumption significantly below the global average-especially among the Muslim-majority countries (Maldives, Indonesia, and Malaysia)-the majority of the countries in this region had markedly increased their alcohol consumption along with the economic development they experienced between 2010 and 2017. In fact, five middle-income countries in this region (Vietnam, Lao PDR, Cambodia, Myanmar, and Timor-Leste) were in the top 12 countries globally based on absolute increases in adult alcohol per capita consumption and apply an appropriate excise tax on alcohol products with regular increases to reflect inflation.Large bowel obstruction (LBO) is associated with high morbidity and mortality due to delayed diagnosis and/or treatment. MDCT has become the standard of care to identify the site, severity, and etiology of obstruction. The goal of this review is fourfold. The first objective is to give clues to differentiate LBO from colonic pseudo-obstruction. The second objective is to describe CT features in the most common cause of LBO which is colonic cancer by illustrating classical and atypical features of colonic cancer responsible for LBO and by giving the features which must be reported when differentiating malignant from benign presence of local lymph nodes, other colic localizations, length of involved segment, presence of diverticula, or other. The third objective is to illustrate the various causes of LBO which can mimic a colon cancer by leading to a thickening of the colonic wall diverticulitis, ischemic colitis, endometriosis, inflammatory disease and to give tips which permit to evoke another diagnosis than a colon cancer in patient with a LBO and a thickening of the colic wall. The fourth objective is to describe the common signs of cecal and sigmoid volvulus and to give tips for a diagnosis sometimes difficult particularly for cecal volvulus one of two transition points according to the type of volvulus and the presence of a whirl sign with a torsion of the mesenteric vessels.Cancer nanotheranostic materials are helpful in monitoring drug delivery and efficacy against tumor cells. Current chemotherapeutic may have adverse side effects and this necessity to discover the new modern therapeutic nano-drugs. In the present study, we designed the new targeted and degradable polymer of bio-active chitosan nanoparticles with proanthocyanidin (PAC-CSNPs) and evaluated its apoptotic effects against human colorectal carcinoma cells (HT-29). The functional groups were characterized by Fourier-transform infrared spectroscopy and transmission electron microscope. Further, their dispersion of spherical form nanoparticle with an average size of 73.43 nm used for drug delivery system. The PAC-CSNPs were targeted to inhibit the cyclin-dependent kinases and prevent cell cycle/cell division in cancer cells. At high concentrations of PAC (25 μg/mL) exposure, cell viability of HT-29 cells was greater than 80%. However, at low concentrations of PAC-CSNPs (6.25 μg/mL) exposure, HT-29 cell mortality was high, which may be due to the efficient drug release by CSNPs.  https://www.selleckchem.com/products/azd3229.html  The percentage of reactive oxygen species (ROS) levels were 12 ± 2.52% (control), 39 ± 4.32% (PAC), and 85.06 ± 3.54% (PAC-CSNPs). The over production of ROS by PAC-CSNPs can prompt DNA damage, cell death and apoptosis in HT-29 cells. The in vivo toxicity of synthesized PAC-CSNPs was tested against zebra fish observed at dose-time-dependent intervals. In conclusion, the PAC-CSNPs enhanced HT-29 cell death and shows promise as a novel future nano-therapy for cancer.The fate of extracellular antibiotic resistance genes (eARGs) in waste activated sludge during anaerobic digestion (AD) remained unclear. The current study investigated the changes in seven eARGs (sulI, sulII, tet(A), tet(O), tet(X), blaTEM, and blaSHV) and intI1 during sludge AD at 35 °C and 55 °C. First, the extracellular DNA (eDNA) extraction method from sludge was optimized by adding sodium dodecyl sulfonate, and the eDNA recovery nearly doubled. Second, analysis via qPCR showed that eARGs ranged from 1.5% to 85.1% of the total ARGs, stressing the importance of eARGs in sludge. Besides, the abundances of all detected eARGs decreased following AD, where removal rates ranged from 22.8% to 93.9% at 35 °C and 52.7% to 96.6% at 55 °C. Further analysis showed that the removal rates of eARGs were negatively correlated with their initial abundances (P less then 0.05). Last, the degradation characteristics of eARGs under AD conditions were determined. The first-order degradation rate constants for different eARGs did not vary significantly, indicating that gene sequences did not cause a removal distinction, and fitted Michaelis-Menten equation confirmed the higher eARGs degradation ability at the higher temperature.