https://www.selleckchem.com/products/rgfp966.html Autophagic activity of platelets correlated negatively with the efficacy of clinical platelet transfusion. These research findings provide a theoretical basis for effective clinical platelet transfusion. Noninvasive prenatal testing (NIPT) for fetal antigens is a common standard for targeted immune prophylaxis in RhD-mediated hemolytic disease of the fetus and newborn, and is most frequently done by quantitative PCR (qPCR). A similar approach is considered for other blood group and human platelet alloantigens (HPA). Because of a higher sensitivity compared to qPCR for rare molecule detection, we established and validated digital PCR (dPCR) assays for the detection of exons 3, 5 and 7, KEL1, HPA-1a, and HPA-5b from cell-free DNA (cfDNA) in plasma. The dPCR assays for the Y-chromosomal marker amelogenin and autosomal SNPs were implemented as controls for the proof of fetal DNA. Validation was performed on dilution series of mixed plasma samples from volunteer donors with known genotypes. After preamplification of the target loci, two-color (FAM and VIC) TaqMan<sup>TM</sup> probe chemistry and chip-based dPCR were applied. The assays for included as an internal control. For the diallelice assays, the NIPT protocol for RhD, KEL1, and HPA can also be applied to earlier stages of pregnancy. Analysis of cfDNA from maternal plasma using dPCR is suitable for the detection of fetal alleles. Because of the high sensitivity of the assays, the NIPT protocol for RhD, KEL1, and HPA can also be applied to earlier stages of pregnancy. Individuals with the CisAB phenotype are rare in the Chinese population. In the present study, we investigated the sequence of the gene and family members of a newborn suspected to have the CisAB phenotype. The ABO phenotype was detected using conventional serological tests. The full coding region of exons 1 to 7 of the gene was amplified by polymerase chain reaction and was sequenced. The haplotype was