https://www.selleckchem.com/products/Ubenimex(Bestatin).html Our results indicate that expression of mEGFP-tagged Nt-RanBP9 followed by thrombin cleavage of the tag was the most effective method to obtain the protein with >90% purity and good yields. We report and discuss the challenges and optimal methods to obtain the N-terminal region of RanBP9, a protein with functional implications in multiple biological processes and neurodegenerative diseases. OBJECTIVE To determine baseline knowledge of emergency medicine (EM) residents at 2 different residency training programs and assess if a 4-week orthopedic surgery rotation would improve musculoskeletal (MSK) knowledge as assessed by the basic competency exam (BCE). This study also sought to compare variations of the BCE to determine how emergency physicians would perform on the full 25-question assessment vs a modified 18-question test. DESIGN Residents from 2 different EM residency training programs were given the BCE to determine baseline MSK knowledge prior to their orthopedic surgery rotations. A postrotation BCE was given to the residents from both EM training programs upon completion of their orthopedic surgery rotation. Both prerotation and postrotation tests were reviewed and scored independently by a panel of experts and later assessed by 2 independent reviewers. SETTING San Antonio Military Medical Center, SA Tx Level I Trauma, Tertiary Care Center University of Texas Health, SA, Tx, Level 1 Trauma, Tertiary Care Center. PARTICIPANTS A total of 54 EM residents completed both the prerotation test and pos-rotation test. RESULTS EM residents at both programs had significantly improved test scores after an orthopedic surgery rotation. Baselines scores of PGY-2 residents were higher than PGY-1 residents. CONCLUSIONS EM residents can improve their competency in MSK education with a 4-week rotation in orthopedic surgery. Further studies are needed to determine knowledge retention and to identify components of an opt