003), DIC (p = 0.018), and severe neonatal morbidity (p  less then  0.001). The late PA group was characterized by a higher rate of urgent Cesarean deliveries (p  less then  0.001). The early PA group was characterized by higher rates of placental maternal vascular malperfusion (MVM) lesions (p  less then  0.001), maternal inflammatory response (MIR) lesions (p  less then  0.001), placental hemorrhage (p  less then  0.001), and a lower feto-placental ratio (p  less then  0.001). Using regression analysis, we found that severe neonatal morbidity was independently associated with early abruption (aOR = 5.3, 95% CI = 3.9-7.6), placental MVM (aOR = 1.5, 95% CI = 1.2-1.9), placental MIR (aOR = 1.9, 95% CI = 1.4-2.3), and inversely associated with antenatal corticosteroids (aOR = 0.9, 95% CI = 0.6-0.98). "Early" and "late" PA significantly differ in their presentation, placental pathology, and pregnancy outcomes.Betulin (BE) can be obtained from many plants, such as those belonging Betulaceae family, and pharmacological investigations showed its notable biological properties and good potential for food and pharmaceutical development. We investigated the homogeneity, stability, purity, and uncertainty of a newly certified reference material (CRM) of BE. The certified purity value for the CRM of BE was 99.56% with an extended uncertainty of 0.07% (k = 2, P = 0.95), as determined by differential scanning calorimetry (DSC). In this study, DSC was used for the first time for purity determination of BE. Given its high accuracy, precision, and reproducibility, DSC can be used as an alternative technique for purity determination of CRMs in the pharmaceutical and food industry.Bivalve molluscs can acquire marine biotoxins by filter-feeding upon certain toxin-producing microalgae. The two most common syndromes observed in temperate coastal waters have been diarrhetic shellfish poisoning (DSP) and paralytic shellfish poisoning (PSP). While DSP is a non-fatal gastrointestinal syndrome, PSP is a neurological syndrome which can lead to death by respiratory paralysis in high intoxication scenarios. In Portugal, the presence of both DSP and PSP toxins leads to recurrent seasonal bans of bivalve harvesting. On a few occasions, the bans were not placed in time, not properly disseminated to the public or were disregarded by recreational harvesters. Several cases of poisonings have been studied in collaboration between health authorities and the laboratory in charge of the biotoxin monitoring programme. Some of the outbreaks have even called the attention of the local media. In several of these recorded cases, a common trait has emerged throughout the years bivalve harvest had often been done during very low tides attributed to either new or full moons. These tides expose intertidal bivalves more widely, increase harvesting time, and allow picking of larger-sized specimens. In some occasions, the consumers were extremely unfortunate because a noxious coincidence had occurred larger-sized specimens were available but had attained the highest toxin content of the toxic season. This review alerts that despite costly monitoring programmes have been perfected through the years, human poisonings still take place due to the rapid increase in toxin levels and/or disrespect of harvest bans.Exposure to PM2.5 can cause adverse health outcomes. In this study, we analyzed PM2.5 samples collected from suburban and urban sites, including a traffic tunnel in Suzhou, China, for their physicochemical properties, endotoxin contents, and effects on HepG2 and A549 cells in vitro. The greatest cellular responses, including oxidative stress, cytotoxicity, genotoxicity, inflammatory, and transcriptional activation of stress-responsive genes (i.e., HSPA1A, GADD45α), were observed in cells treated with traffic tunnel PM2.5. Cytokine expression was also measured and closely correlated with endotoxin content, while other toxic effects were largely related to PM2.5-bound metals and polycyclic aromatic hydrocarbons (PAHs). These findings suggested that chemical and biological composition of PM2.5, including adsorbed trace metals, PAHs, and endotoxin, may contribute significantly to their toxicity. In addition to commonly used in vitro toxicity tests, HSPA1A and GADD45α promoter-driven luciferase reporter cells may provide a potential new tool for rapid screening and quantification of PM2.5 toxicity.Excessive doses of toxic metals such as cobalt may cause detrimental hazards to exposed organisms. Six groups of onion bulbs were formed to investigate the therapeutic effects of grape seed extract (GSE) against cobalt(II) nitrate (Co(NO3)2) exposure in Allium cepa L. root tips. Control group was irrigated with tap water, while the latter groups were exposed to 150 mg/L GSE, 300 mg/L GSE, 5.5 ppm Co(NO3)2, 5.5 ppm Co(NO3)2 + 150 mg/L GSE and 5.5 ppm Co(NO3)2 + 300 mg/L GSE, respectively. Co(NO3)2 treatment seriously inhibited the root growth, germination and weight gain of the bulbs. Mitotic index was significantly decreased, whereas the chromosomal aberrations and micronuclei incidence exhibited a remarkable increase. In addition, Co(NO3)2 induced a variety of anatomical disorders in onion roots. Lipid peroxidation levels of the cellular membranes were assessed measuring the malondialdehyde content (MDA).  https://www.selleckchem.com/  MDA amount in Co(NO3)2-treated group reached the highest level among all groups. Co(NO3)2 treatment enhanced the activity of superoxide dismutase and catalase. The addition of GSE to Co(NO3)2 solution substantially suppressed the negative effects of Co(NO3)2 in a dose-dependent manner by strengthening the antioxidant defence system and reducing the cytotoxicity. Moreover, there was a significant recovery in growth parameters following the grape seed addition to Co(NO3)2. GSE had a remarkable reduction in genotoxicity when treated as a mixture with Co(NO3)2. Overall data obtained from this investigation proved that GSE, as a promising functional by-product, had a protective effect on Allium cepa L. against the toxic effects of Co(NO3)2.