https://www.selleckchem.com/products/2-Methoxyestradiol(2ME2).html Focal cortical dysplasia (FCD) is a major cause of drug-resistant epilepsy; however the underlying epileptogenic mechanisms of FCD metabolism in epilepsy patients remain unclear. The aim of this study is to detect alterations of γ-aminobutyric acid (GABA), glutathione (GSH), and the composite of glutamate and glutamine (Glx) in MRI-typical and neuropathologically confirmed FCD-associated epilepsy using Hadamard Encoding and Reconstruction of Mega-Edited Spectroscopy (HERMES). Fourteen epileptic patients suspected to be caused by FCD and 14 healthy controls were enrolled prospectively in this study; all subjects underwent a 3T MRI scan, including 3D T1 weighted imaging and HERMES. The GABA signal detected by HERMES also contains signals from macromolecules and homocarnosine, so it is referred as GABA+. Signals of GABA+, GSH and Glx detected by HERMES from tumor foci, contralateral cerebral regions, and healthy controls were quantified using Gannet. Fitting errors and signal to noise ratios (SNRs) of GABA+signals were also recorded. Differences of GABA+, GSH, Glx, fitting error and SNR of GABA+among three groups were analyzed using linear mixed effects models. Twelve FCD-associated epilepsy patients (7 females, aged 21.9±9.3years) and 12 matched healthy controls (7 females, aged 22.8±9.8years) were finally enrolled in this study. ANOVA results indicated that GABA levels were significantly increased in FCD foci compared with contralateral regions (p=0.008) and with healthy controls (p=0.003), while no difference was found in GSH and Glx levels. No difference of fitting errors or SNR of GABA+was found among FCD foci, contralateral regions and healthy controls. Increased GABA levels were found in FCD foci that indicated GABA may play a central role in the pathophysiology of FCD patients with epilepsy. Increased GABA levels were found in FCD foci that indicated GABA may play a central role in the pathophys