https://www.selleckchem.com/products/mpi-0479605.html The passive nature leads to the reduction in the available actuator-sensor (AS) pairs while the directionality makes the signal processing a challenge. The proposed two-step methodology overcomes these shortcomings of FBG sensors. In the first step the amplitude weighted elliptical approach is used to identify the hotspots due to the inadequate number of AS pairs, the elliptical approach is not sufficient for damage localization. Therefore, in order to further localize the damage the edge reflection based ray-tracing approach is implemented in the second step. Through the two step method, the damage is accurately located. The paper provides the proof of concept of the proposed methodology on an aluminum plate with simulated damage. The results indicate, that indeed the two-step methodology allows accurate damage localization and overcomes the possibility of false detections.The effective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a collagen matrix (CM) for tissue engineering in the chorioallantoic membrane (CAM) assay, which is performed with fertilized chicken eggs. CM were placed on the CAM on embryonic development day (EDD) 7; at EDD-10, ESWT was conducted at 0.12 mJ/mm2 with 500 impulses each. One and four days later, angiogenesis represented by vascularized area, vessel density, and vessel junctions as well as HIF-1α and VEGF gene expression were evaluated. Furthermore, immune response (iNOS2, MMP-9, and MMP-13 via qPCR) was assessed and compared between ESWT- and non-ESWT-groups. At EDD-14, the vascularized area (+115% vs. +26%) and the increase in vessel junctions (+751% vs. +363%) were significantly higher in the ESWT-