The accuracies of the models' results were verified through graphical and statistical analyses. RBF-NN performance is promising and surpassed that of other models in estimating the CO2 loading capacities of AAs aqueous solutions.Different cellular processes that contribute to protein production in Chinese hamster ovary (CHO) cells have been previously investigated by proteomics. However, although the classical secretory pathway (CSP) has been well documented as a bottleneck during recombinant protein (RP) production, it has not been well represented in previous proteomic studies. Hence, the significance of this pathway for production of RP was assessed by identifying its own proteins that were associated to changes in RP production, through subcellular fractionation coupled to shot-gun proteomics. Two CHO cell lines producing a monoclonal antibody with different specific productivities were used as cellular models, from which 4952 protein groups were identified, which represent a coverage of 59% of the Chinese hamster proteome. Data are available via ProteomeXchange with identifier PXD021014. By using SAM and ROTS algorithms, 493 proteins were classified as differentially expressed, of which about 80% was proposed as novel targets and one-third were assigned to the CSP. Endoplasmic reticulum (ER) stress, unfolded protein response, calcium homeostasis, vesicle traffic, glycosylation, autophagy, proteasomal activity, protein synthesis and translocation into ER lumen, and secretion of extracellular matrix components were some of the affected processes that occurred in the secretory pathway. Processes from other cellular compartments, such as DNA replication, transcription, cytoskeleton organization, signaling, and metabolism, were also modified. This study gives new insights into the molecular traits of higher producer cells and provides novel targets for development of new sub-lines with improved phenotypes for RP production.Metallurgical coke gasification by carbon dioxide was kinetically investigated through the use of thermogravimetric analysis under nonisothermal conditions. The results showed that the activation energy, gained by the Cai-Chen iterative model-free method, was estimated to be 183.15 kJ·mol-1. Notwithstanding, the mechanism function f(α) cannot be directly determined due to the fact that f(α) and the pre-exponential factor A α were lumped together as [A α f(α)]; this situation may be tackled by means of the master-plot methods. The most probable mechanism function, determined by the Málek master-plot method (based on Z(α) master plots), was discovered to be the Johnson-Mehl-Avrami equation.  https://www.selleckchem.com/products/alkbh5-inhibitor-2.html  The usefulness of the compound kinetic calculation technique founded upon complemental application of the Málek master-plot and Cai-Chen model-free methods in estimating reaction kinetics of metallurgical coke gasification was verified. The comparison between original and reconstructed kinetic curves judged the accuracy of the gained kinetic parameters. By means of gained nonisothermal kinetic results, the forecasting of kinetic curves in isothermal as well as nonisothermal conditions was performed. In this work, new kinetic equations were presented and applied to reproducing and forecasting kinetic curves.Nanocellulose-assisted gold nanoparticles are considered promising materials for developing eco-friendly diagnostic tools for biosensing applications. In this study, we synthesized 2,2,6,6-tetramethylpiperidin-1-piperidinyloxy (TEMPO)-oxidized cellulose nanocrystal (TEMPO-CNC)-capped gold nanoparticles (AuNPs) for the colorimetric detection of unamplified pathogenic DNA oligomers of methicillin-resistant Staphylococcus aureus. The fabricated TEMPO-CNC-AuNPs (TC-AuNPs) were characterized using UV-visible spectroscopy, transmission electron microscopy, atomic force microscopy, and dynamic light scattering. The average diameter of the synthesized AuNPs was approximately 30 nm. The aqueous solution of TC-AuNPs was stable and exhibited an absorption peak at 520 nm. The chemical interaction between TC-AuNPs and the surface charge of the target and non-target DNA determined the colorimetric differences under ionic conditions. A dramatic color change (red → blue) was observed in the TC-AuNP solution with the target DNA under ionic conditions due to the aggregation of AuNPs. However, no observable color change occurred in the TC-AuNP solution with the non-target DNA under similar conditions owing to the better shielding effects of the charged moieties. The colorimetric detection limit of the TC-AuNPs was demonstrated to be as low as 20 fM pathogenic DNA. Therefore, the use of TEMPO-oxidized CNC-capped AuNPs is efficient and straightforward as a biosensor for the colorimetric detection of pathogenic DNA.Green synthesis, based on green chemistry, is replacing the traditional methods, aiming to contribute with an enhanced environmental sustainability, which can be achieved using nontoxic compounds from biological resources, such as natural extracts from plants. In this study, the life cycle assessment (LCA) of iron oxide nanoparticles prepared through the green synthesis and the coprecipitation method is reported by following a cradle-to-gate approach. The LCA allowed quantifying and normalized the environmental impacts produced by the green synthesis (1.0 × 10-9), which used a Cymbopogon citratus (C. citratus) extract and sodium carbonate (Na2CO3). The impacts were also determined for the coprecipitation method (1.4 × 10-8) using the iron(II) salt precursor and sodium hydroxide (NaOH). The contribution of C. citratus extract and Na2CO3 as the precursor and pH-stabilizing agents, respectively, was compared regarding the iron(II) and NaOH compounds. Environmental sustainability was evaluated in human toxicity, ecosystem quality, and resource depletion. The major environmental contribution was found in the marine aquatic ecotoxicity (7.6 × 10-10 and 1.22 × 10-8 for green synthesis and the coprecipitation method) due to the highest values for ethanol (3.5 × 10-10) and electricity (1.4 × 10-8) usage since fossil fuels and wastewater are involved in their production. The C. citratus extract (2.5 × 10-12) presented a better environmental performance, whereas Na2CO3 (4.3 × 10-11) showed a slight increase contribution compared to NaOH (4.1 × 10-11). This is related to their fabrication, involving toxic compounds, land occupation, and excessive water usage. In general, the total environmental impacts are lower for the green synthesis, suggesting the implementation of environmentally friendlier compounds based on natural sources for the production of nanomaterials.