6083 fM, good specificity in the face of single nucleotides and other microRNAs, satisfactory stability and reproducibility for practical analysis. Furthermore, the clinical applicability for circulating miR-21 detection was verified in human serum samples without additional treatment. We hope that this elaborated biosensor will provide new opportunities for bioassays based on DNA nanomaterials.An upright GO (UGO) modified screen-printed electrode was prepared with the help of the external magnetic field for improving its electrochemical performance. The ratio of GO Nafion and the magnetic field intensity on the properties of UGO were examined by scanning electron microscope, cyclic voltammetry and electrochemical impedance spectroscopy. The magnetic field intensity does not influence the electron transfer kinetics but increase the number of active sites and therefore enhance the electroactive surface area. In addition, the UGO electrode that was electrodeposited Ni nanoparticles (denotes as Ni NPs/UGO modified electrode) display excellent oxidation towards glycine using chronoamperometry. The Ni NPs/UGO modified electrode indicated an excellent performance for electrochemical COD (chemical oxide demand) analysis with a linear detection range of 0.1-400 mg/L and a lower detection limit of 0.02 mg/L. Moreover, this Ni NPs/UGO modified electrode can be applied to the rapid determination of COD in general real water samples. The results were in agreement with those obtained by using the standard method (ISO 6060).A procedure for the size characterization and quantification of titanium dioxide (TiO2) nano- and microparticles by Asymmetric Flow Field-Flow Fractionation (AF4) coupled to Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is described.  https://www.selleckchem.com/products/zidesamtinib.html  Different strategies for size characterization with size standards and the use of the DLS signal for the estimation of hydrodynamic diameters are evaluated. The procedure has been applied to the characterization of TiO2 nanoparticles in photocatalytic products and crab sticks (surimis), where TiO2 is present as E171 food additive. Sizes in the range of 50-90 nm and 160-170 nm were estimated in the different photocatalytic products by AF4-DLS, in good agreement with the sizes predicted by calibration versus SiO2 and polystyrene standards. In surimis, sizes between 140 and 350 nm were estimated by AF4-DLS, similar to those reported in literature for E171 additive. These results were also compared to those obtained by single particle ICP-MS, which allowed the detection of a nano-sized fraction of TiO2 present in the four surimis analyzed. Titanium contents in one of the photocatalytic products determined by AF4-ICP-MS was 16.86 ± 2.54 mg g-1, whereas the alkaline extraction followed by AF4-ICP-MS allowed the determination of TiO2 content in four surimis at concentration levels in the range of the μg g-1 (from 3.14 ± 0.10 to 14.55 ± 1.46 μg Ti g-1), with channel recoveries above 85% in all cases. The method has been validated by comparison with the Ti content determined by ICP-OES after microwaved assisted acid digestion of all the samples. The methodology proposed allows the complete quantification of the (nano)particulate forms of titanium in complex matrices together with their size characterization.Highly sensitive and selective detection of DNA adenine methylation methyltransferase (Dam MTase) activity is essential for clinical diagnosis and treatment as Dam MTase can catalyze DNA methylation and has a profound effect on gene regulation. In this study, a fluorescence biosensor has been developed for label-free detection of Dam MTase activity via methylation-sensitive cleavage primers triggered hyperbranched rolling circle amplification (HRCA). A hairpin DNA probe (HP) with a Dam MTase specific recognition sequence on the stem acting as a substrate has been designed. This substrate probe can be methylated by the target in the system and subsequently cleaved by DpnI, which results in the release of the primer release probe (RP) and hence in turn triggers the subsequent HRCA reaction. As the HRCA products contain many double-strand DNA (dsDNA) with different lengths, and the SYBR Green I can be embedded in the dsDNA to produce a strong fluorescence signal. However, in the absence of the target, the presence of the probe HP in the form of a hairpin cannot induce the HRCA reaction, and only weak fluorescence intensity can be detected. Under the optimized conditions, the fluorescence of the system has a linear relationship with the logarithm of the concentration of Dam MTase in the range of 2.5-70 U/mL with a detection limit of 1.8 U/mL. The Dam MTase can be well distinguished from other MTase analogs. The developed sensor was applied to detect target in serum and E. coli cell lysate, and the standard recovery rates were in the range of 96%-105%. The results showed that this method has great potential for assessing Dam MTase activity in complex biological samples. In addition, the method has been applied to detect the related inhibitors with high efficiency.Sangguayin preparation (SGY-P) is refined from the traditional Chinese medicinal compound Sangguayin, which "clears heat and promotes fluid" and "tonifies kidney and spleen" for "Xiaoke", commonly known as 'Diabetes mellitus' in clinics. Previous studies have shown that SGY-P could reduce insulin resistance and repair damaged pancreas in db/db mice, but the underlying mechanisms were unclear. Here, we investigated whether treatment with SGY-P could protect pancreatic β-cells from apoptosis and uncovered the underlying mechanisms. db/db mice were used to observe the hypoglycemic and islet protective effect in vivo. Apoptosis was induced in mouse insulinoma 6 (MIN6) cells by palmitate, following which the cells were treated with SGY-P for elucidating the anti-apoptotic mechanism in vitro. Cell viability and nuclear morphology were detected by CCK-8 assay and Hoechst 33258 staining. The expression levels of apoptosis-, endoplasmic reticulum (ER) stress-, and autophagy-related proteins were measured by western blot.