https://www.selleckchem.com/products/z-lehd-fmk-s7313.html 5% (95% confidence interval [CI]26.0-35.2) and 8.3% (95% CI 7.0-9.8), respectively. The quarter level isolation rate of species was 38.6% (95% CI 30.1-47.6). Five species namely , and were identified. The latter two are CNS and were identified for the first time in Ethiopia. Antimicrobial susceptibility testing showed none of the isolates of , 100% of , and 87.5% of species were multidrug-resistant. The independent predictors of mastitis (p<0.05) were the age of the cows, stage of lactation, type of housing, the interval of bedding cleaning, and previous history of mastitis. The study showed a high prevalence of mastitis, species, and multidrug resistant , and in Zebu cows. The study showed a high prevalence of mastitis, Staphylococcus species, and multidrug resistant S. lentus, and S. sciuri in Zebu cows. The ability of to form biofilms is associated with high mortality and treatment costs. Established biofilms cannot be eradicated by many conventional antibiotics due to the development of antibiotic tolerance by . Here we report the synthesis and biological characterization of novel small-molecule compounds with antibiofilm activity. Chromone 5-maleimide substitution compounds (CM3a) showed favorable antibacterial activity against . CM3A with antibacterial activity was synthesized and screened. The minimum inhibitory concentration (MIC) of CM3a were determined by the broth microdilution method. Biofilm eradication assay and colony count methods were used to investigate the effect of CM3a on biofilm disruption and killing. Changes in biofilm architecture when subjected to CM3a, were visualized using confocal laser scanning microscopy (CLSM). CCK-8 assay and survival rate of larvae were used to test the toxicity of CM3a. The minimum inhibitory concentration (MIC) of CM3a against was about 26.4 μM. Biofilm staining and laser scanning confocal microscopy analysis showed that CM3a eradicated biofilms by reducin