https://www.selleckchem.com/products/ABT-888.html Mitochondria possess a small genome that codes for core subunits of the oxidative phosphorylation system and whose expression is essential for energy production. Information on the regulation and spatial organization of mitochondrial gene expression in the cellular context has been difficult to obtain. Here we devise an imaging approach to analyze mitochondrial translation within the context of single cells, by following the incorporation of clickable non-canonical amino acids. We apply this method to multiple cell types, including specialized cells such as cardiomyocytes and neurons, and monitor with spatial resolution mitochondrial translation in axons and dendrites. We also show that translation imaging allows to monitor mitochondrial protein expression in patient fibroblasts. Approaching mitochondrial translation with click chemistry opens new avenues to understand how mitochondrial biogenesis is integrated into the cellular context and can be used to assess mitochondrial gene expression in mitochondrial diseases.The tyrannosaurids are among the most well-studied dinosaurs described by science, and analysis of their feeding biomechanics allows for comparison between established tyrannosaurid genera and across ontogeny. 3D finite element analysis (FEA) was used to model and quantify the mechanical properties of the mandibles (lower jaws) of three tyrannosaurine tyrannosaurids of different sizes. To increase evolutionary scope and context for 3D tyrannosaurine results, a broader sample of validated 2D mandible FEA enabled comparisons between ontogenetic stages of Tyrannosaurus rex and other large theropods. It was found that mandibles of small juvenile and large subadult tyrannosaurs experienced lower stress overall because muscle forces were relatively lower, but experienced greater simulated stresses at decreasing sizes when specimen muscle force is normalized. The strain on post-dentary ligaments decreases stres