https://www.selleckchem.com/Proteasome.html BACKGROUND Rhaphidophora pinnata (L.f.) Schott is one of the Indonesian plants, has known as a medicinal plant. This plant is a vine; round stems have sticky roots and hanging roots. The leaves have been used as traditional anticancer in Singapore. Indonesian people have also used R. pinnata plants as a diuretic agent, anticancer and antibacterial. R. pinnata plants contain active substances from alkaloid compounds, flavonoids, saponins, tannins and triterpenoids/steroids. AIM The purpose of this study was to determine the organic and inorganic content of Rhaphidophora pinnata (L.f.) Schott water extract in the form of fresh leaves, micro simplicia and nano simplicia. METHOD The collected R. pinnata leaves are drained and grinded to make micro, and nano Simplicia powder of R. pinnata leaves. The size characterisation of R. pinnata leaves was analysing using Particles Size Analyzer. The water extract of R. pinnata leaves, micro simplicia, and nano simplicia R. pinnata leaves was made 10% (w / v) in water. Phytcontent than other water extracts on the TLC plate by detection at a wavelength of 250 nm and 300 nm. The most element content in R. pinnata water extract is potassium, followed by magnesium, sodium, and calcium. Copyright © 2019 Masfria Masfria, Marianne Marianne, Yade Metri Permata.BACKGROUND The application of nanotechnology is aimed to enhance the capability of the chemical compounds of Plectranthus amboinicus (Lour.) Spreng. leaves activity. The contact area of the particle surface becomes larger in nano size, which can increase the amount of the isolated active substance. The nanoparticles of this extract exhibited the most effective impact in decreasing the cell number of T47D by induction of apoptosis and arresting the cell cycle. AIM To evaluate the expressions of cyclin D1, caspase-9 and p53 in T47D cell lines treated by Plectranthus amboinicus, (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles (PAEEN).