https://www.selleckchem.com/products/-r-s--3-5-dhpg.html Aim To determine changes in global DNA methylation in monocytes from neonates of women with obesity, as markers of an immune programming resulting from maternal obesity. Materials & methods Cord blood monocytes were obtained from neonates born to women with obesity and normal weight, genome-wide differentially methylated CpGs were determined using an Infinium MethylationEPIC-BeadChip (850K). Results No clustering of samples according to maternal BMI was observed, but ***-specific analysis revealed 71,728 differentially methylated CpGs in female neonates from women with obesity (p less then 0.01). DAVID analysis showed increased methylation levels within genes involved in the innate immune response and inflammation. Conclusion Maternal obesity induces, in a ***-specific manner, an epigenetic programming of monocytes that could contribute to disease later in life. Clinical trial registry This study is registered in ClinicalTrials.gov NCT02903134.Aim This study investigated the effect of sodium citrate on the properties and biological activity of plasma rich in growth factors (PRGF). Methods PRGF was obtained from trisodium citrate and plain extraction tubes. Hematological parameters, growth factors' release kinetics from both PRGF clots and their releasates' biological effect on human bone cells were evaluated. Results The platelet enrichment factor, the growth factors' content and the release kinetic of PRGF were similar for both groups. The proliferation, collagen type I synthesis and tissue-nonspecific alkaline phosphatase activity of human osteoblasts showed no statistically significant differences. Conclusion The use of sodium citrate does not influence the composition, the growth factors' release kinetics or the biological effect of PRGF, but it increases its clinical versatility.Aim To analyze the expression of urinary exosome-derived miRNAs (exo-miRs) in kidney recipients on tacrolimus-based therapy. Pat