https://www.selleckchem.com/products/zebularine.html The synthesis of ApoE(133-150)-PNA conjugate using chemo-selective NCL was highly efficient and the conjugate was obtained in high yield. Toward synthesizing trifunctional CPP-ASO conjugates, we subsequently conjugated different functional moieties including a phosphorodiamidate morpholino oligonucleotide (PMO), an additional functional peptide or a fluorescent dye (Cy5) to the thiol that was generated after NCL. The in vitro analysis of the bifunctional CPP-PNA and trifunctional CPP-(PMO)-PNA, CPP-(peptide)-PNA and CPP-(Cy5)-PNA showed that all conjugates are cell-permeable and biologically active. Here we demonstrated chemo-selective NCL as a highly efficient and superior conjugation strategy to previously published methods for facile solution-phase synthesis of bi-/trifunctional CPP-ASO conjugates.Rare earth element recovery in molten salt electrolysis is approximately between 91 and 93%, whereof 8% is lost in waste molten salt slag. Presently, minimal research has been conducted on the technology for recycling waste rare earth molten salt slag, which is either discarded as industrial garbage or mixed with waste slag into qualified molten salt. The development of a new approach toward the effective treatment of rare earth fluoride molten salt electrolytic slag, which can recycle the remaining rare earth and improve the utilization rate, is essential. Herein, weak magnetic iron separation, sulfuric acid leaching transformation, water leaching, hydrogen fluoride water absorption, and cycle precipitation of rare earth are used to recover rare earth from their fluoride molten salt electrolytic slag, wherein the thermodynamic and kinetic processes of sulfuric acid leaching transformation are emphatically studied. Thermodynamic results show that temperature has a great influence on sulfuric acid leaching. Withat increasing sulfuric acid concentration can change the kinetic-control region and strengthen the kinetic pr