https://www.selleckchem.com/ The repeatability precision of independent laboratory testing was 13-27% for orange juice and 16-43% for ham. Interference by several disinfectants indicate that rinsing is recommended to be performed after the use of sanitizing agents and before testing with LuciPac A3 Surface. Selectivity testing revealed that no positive interference and no inhibition were caused by adenylate analogues. Instrument variation, lot-to-lot consistency, accelerated stability (30°C, 5 weeks) were confirmed, and the method was shown to be robust against shaking time. The LuciPac A3 Surface has been successfully validated. This A3 swabbing assay kit was qualified for PTM certification No. 051901. This A3 swabbing assay kit was qualified for PTM certification No. 051901. The CompactDry "Nissui" ETC is a ready-to-use dry media sheet using a chromogenic medium with selective agents for the detection and enumeration of Enterococcus spp. After a 20-24 h incubation at 37 ± 1°C, Enterococcus colonies appear blue. The performance of the CompactDry "Nissui" ETC was compared to that of the Nordic Committee on Food Analysis (NMKL) reference method 68, Enterococcus, Determination in Foods and Feeds, for 10 matrixes including cream, custard, lettuce, parsley, pasta salad, frozen ground beef patties, fresh cooked prawns, sandwiches, raw beef, and tuna pate. Performance indicators included repeatability, difference of means (DOM), robustness and inclusivity/exclusivity. For all 30 materials (10 matrixes each inoculated at three levels), the relative standard deviation of repeatability (RSDr) was <10% for both methods. Most materials yielded RSDr <5%, with five materials between 5 and 10% for the CompactDry "Nissui" ETC method and ten materials between 5 and 10% for the NMKL 68 method. The DOM ranged from -0.240 (95% CI-0.363, -0.118) to 0.228 (95% CI 0.041, 0.415) log10 CFU/g, well within the -0.5 to 0.5 log10 CFU/g range generally accepted as microbiologically insignifican