This study aimed to investigate the effects of 17β-estradiol and estrogen receptors (ERs) in U2OS cells. Osteosarcoma U2OS cells were divided into six groups, and cell proliferation was determined using the cell counting kit-8 growth test. Furthermore, U2OS cell migration and invasion were examined by cell scratch test and Transwell invasion assays, respectively. At 48 h of 17β-estradiol exposure, U2OS cell viability decreased ( <0.001); however, ERα siRNA and ERβ siR-NAs significantly increased cell viability ( <0.01). Considering the cell positions at 0 h, the cell migration distance at 24 h significantly reduced in the presence of 17β-estradiol ( <0.001); however, ERα and ERβ siRNAs significantly increased cell migration distance ( <0.01). The number of invasive cells significantly decreased upon exposure to 17β-estradiol ( <0.001); however, ERα and ERβ siRNAs significantly increased the number of invasive cells ( <0.01 and <0.05, respectively). 17β-estradiol exhibited significant anti-tumor effects on U2OS cells that were mediated by ERs and reduced cell proliferation, migration, and invasion. 17β-estradiol exhibited significant anti-tumor effects on U2OS cells that were mediated by ERs and reduced cell proliferation, migration, and invasion.Macrophages have the potential to re-programing tumor cells in the tumor microenvironments. Thus we investigated anti-cancer effects of M1-polarized macrophages by lipopolysaccharide (LPS) on the physiological properties of human prostate cancer PC-3 cells. To identify communications with immune cells and tumor cells, we performed in-direct way by using conditioned-media (CM) and analyzed tumor properties via quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and western blot and flow cytometry. CM of M1-polarized macrophages induced apoptotic cell death in PC-3 cells, and it surprisingly suppressed tumor parameters including epithelial to mesenchymal transition (EMT), invasion, migration and angiogenesis. EMT specific markers, N-cadherin, snail-1, and TGF β2 were diminished; however, E-cadherin was increased. In addition, migration markers, vimentin and CCL2 were down-regulated, and finally wound healing was also inhibited. Decreased expression of matrix metalloprotein (MMP)-9 and VEGFA might reduce the invasive and angiogenic abilities of PC-3 cells. These results suggested that co-culture with CM of M1-polarized macrophages showed higher anti-cancer effects on PC-3 cells. Thus, therapeutic targeting of macrophages toward PC-3 cells may represent a useful strategy to complement with the secreted molecules of RAW 264.7 cells as inhibitors of metastasis and anti-cancer agents.Hepatocellular carcinoma (HCC) is a high-level invasive and metastatic malignancy with a low survival rate. Accumulating evidence has proved that circular RNAs (cirRNAs) function as potential biomarkers for diagnosis or prognosis in various cancer, including HCC. In the present study, we aimed to explore a novel biomarker, circ_0067934 for diagnosis and prognosis of HCC so as to contribute to therapeutic strategies. In the present study, the expression levels of circ_0067934 in HCC patients and paired controls were determined by real-time quantitative polymerase chain reaction (qRT-PCR) analysis. The correlation between different circ_0067934 expressions with clinicopathological factors of HCC patients was analyzed by one-way analysis of variance (ANOVA). Then, we examined the potential diagnostic value of circ_0067934 by measuring the receiver operating characteristic (ROC) curves. Finally, the overall survival rate evaluated by Spearman's rank correlation coefficient of HCC patients with different circ_0067934 was compared in order to present the prognostic value of circ_0067934 in HCC. From the results, qRT-PCR analysis revealed that circ_0067934 was significantly up-regulated in HCC tissues compared with adjacent non-tumor tissues. Moreover, circ_0067934 positively correlated with tumor size (P=0.0025), TNM stage (P less then 0.0001) and microvascular invasion (P less then 0.0001). However, there were no significant differences in gender, age, family history, diabetes, ALT, AST, AFP and AKP. Meanwhile, the ROC curves presented that circ_0067934 had favorable specificity and sensitivity with the area under the curves of 0.8412 (95% CI=0.7881 to 0.8943). Furthermore, the upregulation of circ_0067934 was associated with unfavorable prognosis in patients with HCC. https://www.selleckchem.com/products/AZD2281(Olaparib).html In conclusion, circ_0067934 may function as a potential biomarker for the diagnosis and prognosis of HCC. Annexin A2 is a calcium dependent phospholipid binding protein that is a biomarker in cancers. However, the value of serum Annexin A2 in the diagnosis of colorectal cancer (CRC) is not clear. This study aimed to investigate clinical utility of serum Annexin A2 as a potential biomarker for CRC. Annexin A2 was analyzed in 20 cases of CRC tissues and 20 controls of normal adjacent paired tissues. Serum Annexin A2 was calculated in 59 CRC patients and 44 healthy subjects. Receiver operating characteristic (ROC) curve and logistic regression were utilized to evaluate the diagnostic effectiveness and construct diagnostic model. Annexin A2 in CRC tissues was slightly higher than in normal adjacent paired tissues (χ2=6.0652, <0.05). Serum Annexin A2 in CRC patients was significantly lower than in healthy controls ( <0.05). Besides, the levels of serum Annexin A2 were lower in patients with poor tumor differentiation than in well or moderate tumor differentiation ( =0.0111). ROC analysis indicated the diagnostic efficacy of serum Annexin A2 was better than carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA199) for CRC. Furthermore, joint detection of Annexin A2 and CEA had the maximum area under the ROC curve (AUC) in discriminating CRC from healthy controls (AUC 0.931, sensitivity 86.4%, specificity 84.7%, positive predictive value 87.9%, and negative predictive value 82.2%). Serum Annexin A2 may be a non-invasive and promising biomarker for the diagnosis of CRC, and the joint detection of Annexin A2 and CEA may have been favorable clinical applied value in the diagnosis of CRC. Serum Annexin A2 may be a non-invasive and promising biomarker for the diagnosis of CRC, and the joint detection of Annexin A2 and CEA may have been favorable clinical applied value in the diagnosis of CRC.