https://www.selleckchem.com/products/chk2-inhibitor-2-bml-277.html The relationships between environmental variables (sO2, dO2, water temp., salinity, flow, TDS, Cond, pH, NO3-N, NO2-N, PO4-P, Fe+3, NH4-N) and bioindicators have been revealed by using multivariate analyses (NMDS, CCA). The region-based variations in water quality were compared with the Kruskal-Wallis test. The one-way variance analysis test (ANOVA) was used for the contrast between the biotic indices. Significant differences (p less then 0.05) were found among the regions in terms of Shannon-Wiener, Evenness, BMWP and MMIF indices. Regions were separated according to pollution sources, and the impact of provinces on water quality may vary according to their industry types. It has been observed that pollutants can spread across a basin for very long distances and reinstatement of the environmental conditions may require long periods. Breast cancer (BC) is the 2 most prevalent malignancy worldwide and is the most prevalent cancer among Egyptian women. The number of newly described cancer-associated genes has grown exponentially since the emergence of next-generation sequencing (NGS) technology. We aim to identify activating mutations in liquid biopsy of Egyptian breast cancer patients using targeted NGS technology. We also demonstrate the microsatellite instability (MSI) status using BAT25, BAT26, and NR27 markers which are tested on the Bioanalyzer 2100 system. Twenty-one variants were detected in 15 genes 7 Substitution-Missense, 12 Substitution-coding silent, and 2 Substitution-intronic. Regarding ClinVar database, out of 21 variants there were 14 benign variants, 3 variants with conflicting interpretations of pathogenicity, 3 variants not reported, and 1 drug response variant. TP53 p.(Pro72Arg) missense mutations were found in 75% of patients. PIK3CA p.(Ile391Met), KDR p.(Gln472His) missense mutations were detected in 25% of pati liquid-based versus tissue-based biopsy and longer follow up period.