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https://www.selleckchem.com/products/mycro-3.html Disease severity in the fungicide-free control was 70%, application of fluxapyroxad+pyraclostrobin decreased disease severity to 18%, followed by pyraclostrobin (23%) and fluxapyroxad (48%). Application of fluxapyroxad+pyraclostrobin also improved seed yield to 2562 kg ha-1 compared with 1874 kg ha-1 for the fungicide-free control, followed by pyraclostrobin (2391 kg ha-1) and fluxapyroxad (2340 kg ha-1). Fungicide application at early and mid-flowering stage had the same effects on disease severity and seed yield; however, seed quality was improved more when fungicide was applied at mid-flowering stage. Continuous use of the same fungicide may result in the development of fungicide insensitivity in the pathogen population. Thus, sensitivity of S. linicola isolates to pyraclostrobin and fluxapyroxad fungicides were determined by the spore germination and microtiter assay methods. Fungicide insensitivity was not detected among the 73 isolates of S. linicola tested against either of these fungicides.The soil-borne pathogen Sclerotinia sclerotorium is the causal agent of sclerotinia stem rot, a severe disease of broad-leaf crops including canola/rapeseed Brassica napus that can result in significant yield losses. Sclerotia, the hard melanized resting structure of the pathogen, requires preconditioning before carpogenic germination can occur. We investigated the effect of pre-conditioning temperature (4°C, 20°C, 35°C, 50°C and field conditions) and duration (0, 30, 60, 120, 179, 240, 301 days) on germination of S. sclerotorium sclerotia collected from five canola fields in the south-western Australian grain-belt. The ecological diversity of each population was characterised using mycelial compatibility groups (MCGs) typing. No response was observed for isolates conditioned at 4°C at any time period indicating chilling is not a preconditioning requirement for these isolates. Sclerotia required preconditioning for a minimu

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