New Paste

Content

https://www.selleckchem.com/products/ms-275.html The 2015/16 Zika virus (ZIKV) epidemic led to almost 1 million confirmed cases in 84 countries and was associated to the development of congenital microcephaly and Guillain-Barré syndrome. More recently, a ZIKV African lineage was identified in Brazil raising concerns about a future outbreak. The long-term consequences of viral infection emphasizes the need for the development of effective anti-ZIKV drugs. In this study, we developed and characterized a ZIKV replicon cell line for the screening of viral replication inhibitors. The replicon system was developed by engineering the IRES-Neo cassette into the 3' UTR terminus of the ZIKV Rluc DNA construct. After in vitro transcription, replicon RNA was used to transfect BHK-21 cells, that were selected with G418, thus generating the BHK-21-RepZIKV_IRES-Neo cell line. Through this replicon-based cell system, we identified two molecules with potent anti-ZIKV activities, an imidazonaphthyridine and a riminophenazine, both from the MMV/DNDi Pandemic Response Box library of 400 drug-like compounds. The imidazonaphthyridine, known as RO8191, showed remarkable selectivity against ZIKV, while the riminophenazine, the antibiotic Clofazimine, could act as a non-nucleoside analog inhibitor of viral RNA-dependent RNA polymerase (RdRp), as evidenced both in vitro and in silico. The data showed herein supports the use of replicon-based assays in high-throughput screening format as a biosafe and reliable tool for antiviral drug discovery. Neurodegeneration is a biproduct of aging that results in concomitant cognitive decline. Physical exercise is an emerging intervention to improve brain health. The underlying neural mechanisms linking exercise to neurodegeneration, however, are unclear. Functional brain network connectivity (FBNC) refers to neural regions that are anatomically separate but temporally synched in functional signalling. FBNC can be measured using functional Magnetic Reson

Optional Paste Settings

Title

Select folder or

Syntax Highlighting

Paste Expiration

Paste Status

Password

Description

Our statistics

Create and share texts quickly and easily 🚀

332,177+

Total pastes

170,422,222+

Total views

119+

Registered users

Our features

Everything you need 😉

Pastes creation

Create multiple posts quickly and easily with our powerful editor.

Folders

Store your pastes in different folders with custom names.

Paste protection

Protect your pastes with password, encryption and captcha.

Multiple syntax languages

We have 150+ syntax languages for your codes.

URL monetization

Earn money by enabling url shorteners in your pastes.

Global settings

Configure each aspect of your pastes globally.

Recent Pastes

Teknik Mendapatkan Situs Judi Online Bisa dipercaya
HTML | 160 | 1 minute ago
Untitled
HTML | 772 | 4 minutes ago
Innovations in Garbage Truck Waste Disposal Technology A Comprehensive
HTML | 0.9K | 6 minutes ago
Anammox bacteria push preset nitrogen reduction in hadal trench sedime
HTML | 0.9K | 6 minutes ago
Watch Orlando - Oklahoma City live stream 19.12.2024
HTML | 763 | 7 minutes ago
All Of The Benefits Of Getting A Regular Massage
HTML | 535 | 7 minutes ago
The effect associated with stress and acculturation for the self-rated
HTML | 885 | 9 minutes ago