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https://www.selleckchem.com/products/Ml-133-hcl.html Almost all the automated chemiluminescence analyzers in the market cannot detect total 25-hy-droxyvitamin D (25-OH VD) in fingertip blood sample due to the limited volume. This study aimed to develop a relatively simple pretreatment method to obtain plasma from the fingertip blood samples with limited volume and to establish and evaluate the performance of a chemiluminescence immunoassay (CLIA) for detecting 25-OH VD in plasma. Fixed volume of plasma was obtained using a special glass capillary to pretreat the fingertip blood. The coated microwells were inserted and the reagents were dispensed into wells in the test strips which can be continuously tested on a small automated chemiluminescence analyzer. The performance of the developed method was evaluated, and the method comparison studies were conducted. The limit of detection (LOD) was 1.38 ng/mL. The intra-assay precision of the anticoagulant whole blood samples with low, medium, high concentrations of 25-OH VD were 5.45%, 8.20%, 4.97%, respectivelyneeds only 30 minutes. The developed method can fully satisfy very busy clinical labs. The developed pretreatment method for fingertip blood and the CLIA method can be used to assess the vitamin D status. The correlation of performance and method with the Roche test was good, and the whole test needs only 30 minutes. The developed method can fully satisfy very busy clinical labs. Thiol/disulfide (SH/SS) homeostasis plays an essential and dynamic role in our body and in various cellular activities and pathways such as cell death, regulation of enzyme activities, mechanisms of transcription and cellular signal transduction. Lung cancer is the most commonly seen cancer type in the adult population. Therefore, prediction of metastases gains importance in this population. We included 150 patients with lung cancer who attended Kirikkale University Medical Faculty Hospital between June 2017 and June 2018. Our purpose was t

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